Coding

Part:BBa_K2114011

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-08)
Revision as of 17:22, 19 October 2016 by WladStr (Talk | contribs) (→‎Characterization)


CotG_G4S_HA_GST

C-terminal fusion of glutathione S-transferase to spore coat gene cotG by a flexible GGGGS linker.


Usage and Biology

Figure 1: Schematic representation of the fusion protein.



















Characterization

I) Surface Localization

Figure 2:













II) Functionality

Figure 3:



















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1315
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 712


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Categories
Parameters
None