Coding

Part:BBa_K2114017

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-10)
Revision as of 16:12, 19 October 2016 by WladStr (Talk | contribs) (→‎Characterization)


aGFPnano_HA_aHelix_cgeA

N-terminal fusion of anti-GFP nanobody to spore crust gene cgeA by an alpha helical linker.


Usage and Biology

Figure 1: Schematic representation of the fusion protein.














Characterization

I) Surface Localization

Figure 2: .














II) GFP binding

Figure 3: .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 681
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None