Reporter

Part:BBa_K2150028

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-13)
Revision as of 05:44, 13 October 2016 by Registry (Talk | contribs)

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pTet+RBS+T7RNAP+Ter+pT7+RBS+tetX-GFP(fusion protein)+DT

In this part, the first promoter pTet is constitutively on, producing T7 RNA polymerase(T7RNAP). T7RNAP can activate the T7 promoter, thus producing the fusion protein in large quantity. The fusion protein(referred to as tetX-GFP) of tetX(a tetracycline-degrading enzyme) and GFP combined with a 3*GGGGS linker is used to report the expression level of tetX.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4703


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Categories
Parameters
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