Protein_Domain

Part:BBa_K105001

Designed by: Manuel Gersbacher   Group: iGEM08_ESBS-Strasbourg   (2008-10-12)
Revision as of 20:46, 19 October 2016 by Nllenwalker (Talk | contribs)

VP16 - eucaryotic activation domain

Originally VP16 is a protein from the herpes simplex virus. It activates the transcription of the late genes of this virus by the RNA-polymerase II of the eucaryotic host.

In artificial systems VP16 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective expression of genes with a promoter containing this bait sequence can be achieved.

Improvement

2016 SZU-China

<br\n> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/eukaryote
Parameters
None