Part:BBa_K1974030

GS linker

Figure 1.GS Linker

Introduction

       The GS Linker(BBa_K1974030) that we provide is a 18 amino acid. It is useful for expressing functional fusion proteins. So we want to use it to concent the spider toxin peptide and snowdrop lectin.

Modifying and Improving the Existed Biobrick

       GS Linker(BBa_K416001) is a 15 amino acid flexible peptide linker protein domain that is useful for creating functional fusion proteins. So we want to use it to concent the spider toxin peptide and snowdrop lectin.

      

In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. Futrhermore, we found no result in the previous history using the Flexible Peptide GS Linker(

BBa_K416001

) from 2010 NYU iGEM team. Therefore, we modified the sequence and added three more amino acids from into the linker. In the result, our improved GS Linker is completely proved by our experiments.

Result and Experiment

       In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins(see Result), so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.

Figure 2.GS linker PCR results.

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]