Coding

Part:BBa_K1985012

Designed by: Hanya Benotmane   Group: iGEM16_Kent   (2016-10-14)
Revision as of 19:28, 19 October 2016 by Hanyab (Talk | contribs) (→‎Validation)

[CsgA-SS-Sup35NM]

This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed. The part contains two segments in one biobrick: CsgA signal sequence and Sup35NM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 671


Usage and Biology

The part was used in pSB1A3.

For more information on biology and usage see part BBa_K1739002

Validation

The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: 2029kB for the plasmid backbone and 973kB for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced.

Figure 1. Agarose gel of the restriction digest of BBa_K1985012 in pSB1C3, with EcoRI and PstI.


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