Measurement

Part:BBa_I20246

Designed by: Jason Kelly, Karen Wong, Nishant Bhat   Group: Endy Lab   (2007-08-15)
Revision as of 15:21, 15 August 2007 by Registry (Talk | contribs)

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A device used to characterize promoters throught measurement of RFP expression

Device is based on I2057 and contains a partial biobrick site (EcoR1, Not1, Spe1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter and measurement device with EcoR1 and Spe1. Parts can be removed after testing, which facilitates the screening of libraries.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal prefix found in sequence at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 15
    Illegal AgeI site found at 595
    Illegal AgeI site found at 707
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None