![](https://parts.igem.org/images/partbypart/icon_measurement.png)
Measurement
Part:BBa_I20246
Designed by: Jason Kelly, Karen Wong, Nishant Bhat Group: Endy Lab (2007-08-15)
A device used to characterize promoters throught measurement of RFP expression
Device is based on I2057 and contains a partial biobrick site (EcoR1, Not1, Spe1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter and measurement device with EcoR1 and Spe1. Parts can be removed after testing, which facilitates the screening of libraries.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal SpeI site found at 15 - 12INCOMPATIBLE WITH RFC[12]Illegal prefix found in sequence at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal SpeI site found at 15 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal SpeI site found at 15
Illegal AgeI site found at 595
Illegal AgeI site found at 707 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |