Device
Part:BBa_K1879001
Designed by: Madeleine Stone Group: iGEM16_Virginia (2016-10-12)
promoter + CBZ cleavage enzyme + terminator
This part contains the CBZ-cleavage enzyme with a promoter (BBa_J23118) and RBS upstream of the coding sequence, and terminator (BBa_J61048) downstream of the sequence to make a fully functioning BioBrick. This BioBrick is used in our biocontainment system to cleave the CBZ protecting group from leucine prior to incorporation of leucine into proteins. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1402
Illegal AgeI site found at 354 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 56
Illegal BsaI.rc site found at 1377
[edit]
Categories
Parameters
None |