Reporter

Part:BBa_K1997004

Designed by: Xinyuan Qiu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 16:24, 16 October 2016 by Qiuxinyuan12 (Talk | contribs)


sGFP-N->Zif268->sGFP-C

This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 688
    Illegal AgeI site found at 772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 775
    Illegal BsaI.rc site found at 508
    Illegal BsaI.rc site found at 989


[edit]
Categories
//awards/part_collection/2016
Parameters
None