Composite

Part:BBa_K2036033

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-30)
Revision as of 09:09, 3 October 2016 by Shareycheng (Talk | contribs)


PP2CA-TTADH1-pCyc-Kozak-SnRK2.2-TTADH1-pCyc

Part of genetic circuit in the eukaryotic version of signal filter ([http://2016.igem.org/Team:HUST-China Details see to HUST-China 2016 wiki]).
We construct this circuit to verify the interaction between PP2CA(BBa_K2036005) and SnRK2.2(BBa_K2036003) especially the inhibiting effect of PP2CA to SnRK2.2

Charactor rating circuit of PP2CA and SnRK2.2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
    Illegal BamHI site found at 661
    Illegal BamHI site found at 806
    Illegal BamHI site found at 918
    Illegal BamHI site found at 1816
    Illegal BamHI site found at 2473
    Illegal XhoI site found at 65
    Illegal XhoI site found at 875
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None