Composite

Part:BBa_K2036017

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-20)
Revision as of 05:43, 29 September 2016 by Shareycheng (Talk | contribs)


CI-TT-pR-RBS-GFP-LVAssrAtag

pR is a lytic promoter with two binding sites OR1* and OR2 * which can be recognized by CI. The circuit is built to characterize CI and pR interaction together with control group: pR-GFp-LVAssrAtag (BBa_K2036016).

Fig1:CI and pR interaction characterization circuits

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1604


[edit]
Categories
Parameters
None