Measurement
Part:BBa_I20245
Designed by: Jason Kelly, Karen Wong, Nishant Bhat Group: Endy Lab (2007-08-15)
A device used to characterize promoters throught measurement of GFP expression
Device is based on E0240 and contains a partial biobrick site (EcoR1, Not1, Spe1) upstream of the RBS. To insert a BB promoter for testing, digest both the promoter and measurement device with EcoR1 and Spe1. This design best simulates spacing in actual construction however, parts cannot be removed after testing.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal XbaI site found at 15 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NotI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal XbaI site found at 15 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal XbaI site found at 15 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 684
[edit]
Categories
Parameters
None |