Device
Part:BBa_K2170050:Design
Designed by: Max Mustermann Group: iGEM16_LMU-TUM_Munich (2016-10-11)
Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2448
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 889
Keywords:
Abbreviations:
Design Notes
Related BioBrick:
- Other versions:BBa_K2170052: Secretory prokaryotic biotin binding receptor with single chain avidin
- Related BioBricks:BBa_K2170051: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide
Cloning details:
- Designed in RFC10
Quality control measures:
Backbone:
- Backbone name: pSB1C3
- Resistance: Cp
- Copynumber: high
Protein coding:
- Protein: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin [Nucleotide 1030 to 2445]-->
- Tag: internal A3C5 Tag
Enzymatic activity:
- none
Cytotoxicity:
- none
Safety notes:
Known and anticipated sefety issues: none
Known and anticipated security issues: none
Source
Source:
- Preexisting BioBrick BBa_I739001; BBa_B0015
- Parts Synthesized by IDT
- Professor Dr. Arne Skerra (Chair biological chemistry of TUM)
Organism:
Genesequence derived from Escherichia coli
- Designed for the following Chassis: procaryotic cells
References
Literature references:
Database references: