Coding

Part:BBa_K2114009

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-08)
Revision as of 14:40, 19 October 2016 by WladStr (Talk | contribs) (Usage and Biology)


aGFPnano_HA_aHelix_cotG

N-terminal fusion of anti-GFP nanobody to spore crust gene cotG by a alpha helical linker.


Usage and Biology

Figure 1: Schematic representation of fusion protein.

This part includes the anti-GFP nanobody (describted by Kubala et al. [Ref]) fused by an alpha helical linker [Ref] to the B. subtilis spore crust gene cotG in order to be displayed on the spore surface. The hemagglutinin epitope tag was included in the fusion construct for convenient detection by specific anti-HA antibodies. The cotG gene was amplified from the genome of B. subtilis and the anti-GFP nanobody was amplified from an expression plasmid. The HA tag and the alpha helical linker were introduced by primer extensions. Both PCR fragments were assembled by Gibson cloning into pSB1C3. The fusion construct can released by XbaI and PstI and cloned alongside with an appropriate promoter into an integration vector for B. subtilis by 3A assembly [REF:BioBrickBox].


Characterization

I) Surface localization











II) GFP-binding


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None