Regulatory

Part:BBa_K2036002

Designed by: Wangjie Liu   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 07:02, 28 September 2016 by Shareycheng (Talk | contribs)


Prd29A
A promoter region upstream Arabidopsis rd29A gene whose expression is induced by dehydration, high-salinity, low-temperature, and abscisic acid (ABA) treatments .Promoter RD29A contains the DRE, DRE/CRT-core motif (A/GCCGAC), and ABRE sequences which are cis-acting elements. So the promoter RD29A can be active by transcription factor and then start downstream genes expression.

Application:


we apply RD29A promoter into our eukaryotic filter .when transcription factor ABF2 is phosphorylated by kinase SnRK2.2 ,the RD29A promoter can be activated and promote the expression of SnRK2.2 and target gene. These make the system form a positive feedback and lead to stable expression of target gene.

Fig1:Eukatyote version of Signal Filter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 153


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Categories
Parameters
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