Generator

Part:BBa_K1896013

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-13)
Revision as of 13:55, 19 October 2016 by Bvh (Talk | contribs)


INP_NC-mSA2 generator

This part was used to express a monomeric Streptavidin protein (mSA2) on the cell surface of E. coli. The the INPNC domain, which consists of the N- and C-terminal domains of a Xanthomonas campestris ice nucleating protein, directs the fusion protein to the outer cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
    Illegal AgeI site found at 1082
    Illegal AgeI site found at 1142
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None