Coding

Part:BBa_K1972008

Designed by: Shuli Liang   Group: iGEM16_SCUT-China_A   (2016-10-14)
Revision as of 12:58, 14 October 2016 by Registry (Talk | contribs)

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dszB with tac promoter induced by IPTG

T7 RNA polymerase will not be expressed without IPTG. When IPTG is added to 1 mM, T7 RNA polymerase will be expressed and if there is protein gene with T7 promoter, it will be greatly expressed by the host cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 267
    Illegal AgeI site found at 1104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 864


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Categories
Parameters
None