DNA

Part:BBa_K1976004:Design

Designed by: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Patrick Kunzmann, Tim Maier   Group: iGEM16_TU_Darmstadt   (2016-10-14)
Revision as of 13:13, 19 October 2016 by Timmaier (Talk | contribs) (→‎Design Notes)


λ-attP site flanked with two bidirectional terminators


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As a source for the attP-sequence BBa_I11023 was used. After further research we realized that this BioBrick is not carrying the exact attachment site of λ-phage. Instead, the sequence corresponds to the attachment site used in the Gateway ® cloning system. We had to change three nucleotides by mutagenesis PCR to receive the accurate λ-phage attP sequence.

Source

Synthesis by iDT

References