Device

Part:BBa_K2172010

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)
Revision as of 03:40, 15 October 2016 by Registry (Talk | contribs)

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Tac Promoter-RBS-GST-Thrombin Protease-TEV-GFP-Terminator

This part is a gene circuit that can be used to test whether it is being expressed or not. It comprises of a ribosome binding site (RBS) for ribosomes to bind, a GST label for purification, a thrombin restriction enzyme cleavage site plus a TEV restriction enzyme cleavage site (for adding other segments) and a GFP detection unit. When transformed into bacteria, e.g. E. coli, it can be used to test whether the bacteria are expressing the part not. Other parts, such as the gene SmCPS1, can be inserted into this part via digestion on the thrombin restriction enzyme cleavage site and the TEV site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 747
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


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Categories
Parameters
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