Part:BBa_K2170001:Design
Secretory eukaryotic biotin binding receptor with enhanced monomeric avidin
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2432
Design Notes
Related BioBrick:
Other versions:BBa_K2170002: Secretory eukaryotic biotin binding receptor with single chain avidin
Related BioBricks:BBa_K2170003: Biotinylated receptor with biotin acceptor peptide combined with a secretory BirA
Cloning details:
Designed in RFC25
Quality control measures:
Test digestion using EcorRI & PstI
Sequencing using VF2
Part was partly sequenced
Backbone:
Backbone name: pSB1C3
Resistance: Cp
Copynumber: high
Protein coding:
Protein: Secretory eukaryotic biotin binding receptor with single chain avidin [Nucleotide 633 to 3314]
Enzymatic activity: none
Cytotoxicity:
not known
Safety notes:
Known and anticipated sefety issues: none
Known and anticipated security issues: none
Intellectual property:
Corresponding part author/authors:
Source
Source:
Parts synthesized by IDT
Organism:
Genesequence derived from Escherichia coli
References
Literature references:
[http://www.nature.com/nmeth/journal/v2/n2/full/nmeth735.html >Chen, I., Howarth, M., Lin, W., & Ting, A. Y. (2005). Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nature methods, 2(2), 99-104.]
Database references: