Composite

Part:BBa_K1955009

Designed by: Yu-chang Ku, Pang-ling Huang   Group: iGEM16_CGU_Taiwan   (2016-10-19)
Revision as of 11:47, 19 October 2016 by Innate immunity (Talk | contribs)


pSB1C3-LacI inducible OVA(Ovalbumin)

In order to prove the OVA(Ovalbumin) sequence which we designed can express protein correctly, we use BL21 competent cell to express the HA/OVA sequence and detect the protein by Western blot analysis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 232
    Illegal BamHI site found at 2336
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1463


[edit]
Categories
Parameters
None