Reporter

Part:BBa_K2060001

Designed by: Cardiff_Wales Team, Geraint Parry   Group: iGEM16_Cardiff_Wales   (2016-09-23)
Revision as of 14:39, 15 October 2016 by Gparry75 (Talk | contribs)


Fluorescent protein mKeima expressed by Arabinose promotor

mKeima is a red-shifted fluorescent protein with Excitation of 440nM and Emission of 616nM. In this part mKeima expression is induced by Arabinose driven by the wildtype pBAD promotor. The red shifted emission spectrum gives it good potential for mammalian cell or tissue bioimaging.

Usage and Biology

We attempted to use arabinose to induce expression of this biobrick variety of concentrations and time periods. Our success with this expression are documented in the Experience section.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None