RNA
Part:BBa_K1941005
Designed by: Dimitri Coukos Group: iGEM16_EPFL (2016-10-13)
scCyc1_MS2
Scaffold RNA that targets the region c3 of CYC1 promoter and recruits the protein repressor module MCP-VP64 via the effector protein recruitments site MS2. MS2 is a well-characterized viral RNA sequence which is recognized by MCP protein. This RNA is composed of the dCas9-binding sgRNA that interacts with CYC1, and the MCP-binding RNA hairpin, MS2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 56
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 170
Illegal NgoMIV site found at 199 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |