RNA

Part:BBa_K1941005

Designed by: Dimitri Coukos   Group: iGEM16_EPFL   (2016-10-13)
Revision as of 20:07, 13 October 2016 by Registry (Talk | contribs)

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scCyc1_MS2

Scaffold RNA that targets the region c3 of CYC1 promoter and recruits the protein repressor module MCP-VP64 via the effector protein recruitments site MS2. MS2 is a well-characterized viral RNA sequence which is recognized by MCP protein. This RNA is composed of the dCas9-binding sgRNA that interacts with CYC1, and the MCP-binding RNA hairpin, MS2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 170
    Illegal NgoMIV site found at 199
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None