Part:BBa_K1913006
434- and lambda cI balance operon + mRFP reporter
This part contains two viral genes: 434- and lambda cI (BBa_C0052 and part of BBa_K081007) expressed under the pBAD/AraC promoter (BBa_I0500). The 434 cI gene is not yet preceded by an RBS, allowing tuning of the cI protein balance. The 434 cI protein encoded in this part contains a C-terminal LVA tag causing more rapid protein degradation, the lambda cI gene in this part does not encode an LVA tag. Additionally this part contains an operon where mRFP (BBa_K081014) is expressed under a modified lambda cI promoter (BBa_I12006). This promoter is (according to the parts page) induced by lambda cI and repressed by 434 cI. This last operon also includes a LVA-tagged lambda cI (BBa_K081007) encoding gene, in theory establishing positive feedback of the modified lambda cI promoter. The two operons in this part were also submitted as separate parts:
- 434- and lambda cI operon: BBa_K1913007
- 434- and lambda cI balance RFP reporter: BBa_K1913016
The complete part is intended to respond with mRFP expression upon a change in strength of the inducible promoter (BBa_I0500). When - after a period of induction with L-Arabinose - the promoter is repressed with glucose, one expects an increase in mRFP (BBa_K081014) expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1898
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 4249
Illegal AgeI site found at 4361 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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