Coding

Part:BBa_K1934070

Designed by: Mathilde Gonthier   Group: iGEM16_INSA-Lyon   (2016-10-13)
Revision as of 11:17, 19 October 2016 by MathildeGonthier (Talk | contribs)


Streptavidin

This part contains the streptavidin coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 376
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 324

Description

Streptavidin is a tetrameric protein of 52.8kDa isolated from the bacterium Streptomyces avidinii.

Streptavidin is used in biotechnologies and especially in diagnostic assays for its very high affinity for biotin. Indeed, this corresponds to a noncovalent interaction that enables an irreversible and very specific bond between biological macromolecules.

BBa_K1934070 contains the coding sequence of streptavidin as a BioBrick part. Be careful, you will need to put this sequence under the control of a promoter, RBS and terminator in order to produce the protein.

Bibliography:

http://www.imiplex.com/resources/53-Streptavidin-Science-ms-cmp.pdf

[1] Fairhead, M., Krndija, D., Lowe, E. and Howarth, M. (2014). Plug-and-Play Pairing via Defined Divalent Streptavidins. Journal of Molecular Biology, 426(1), pp.199-214.

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