Project

Part:BBa_K1926003:Design

Designed by: Xinyi Liu   Group: iGEM16_SYSU-CHINA   (2016-10-09)
Revision as of 04:26, 11 October 2016 by CindyLiu (Talk | contribs) (→‎Design Notes)


A cyclic promoter of CDK4 from human genome


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 522
    Illegal NheI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
    Illegal AgeI site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got the sequence from human genome through PCR using the following primers:

pCDK4-F: TGGTGGAGCGAAAAGGTGACAGCATC

pCDK4-R: GCGGAAACTGGGAGGGCGGGGCGAA

(Product: 457)


The point mutation C→A was done at 221 to remove PstⅠ restriction site.

Source

The sequence was retrieved from Addgene.

References

1. Guo, Z.Y., et al., The elements of human cyclin D1 promoter and regulation involved. Clin Epigenetics, 2011. 2(2): p. 63-76.