Coding
Part:BBa_I718002:Experience
Designed by: David Puyraimond Group: iGEM07_Paris (2007-07-10)
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Applications of BBa_I718002
We transformed DGAT gene in pBluescript SK minus vector (Stratagene) (ampicilline resistance and pLac promoter) into E.coli chemically competent (DH5alpha). We used Nile Red fluorescence dye in the medium (5µg/mL medium) to observe lipid inclusions in different conditions (0.4mM IPTG induction in LB medium with or without sodium oleate 2mM).
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UNIQc1dc8a7781fde080-partinfo-00000000-QINU UNIQc1dc8a7781fde080-partinfo-00000001-QINU