DNA

Part:BBa_K2100030

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-17)
Revision as of 03:22, 19 October 2016 by Sarahcaso (Talk | contribs)


pEXPR pPRE3:eYFP The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.

When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain. The DBD can then bind to the PREs on the promoter, recruiting transcriptional machinery and activating transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 211
    Illegal PstI site found at 466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 211
    Illegal PstI site found at 466
    Illegal AgeI site found at 249
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 225


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Categories
Parameters
None