Coding

Part:BBa_K2066029:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-07)
Revision as of 21:58, 7 October 2016 by Registry (Talk | contribs)

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pBad - TetR/mCherry Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001


Design Notes

Linker and mCherry taken from Synthetic Analog Computation in Living Cells (Daniel et al.). TetR from NCBI NC_011812.1



Source

Codons were optimized for E. Coli expression and non compatible restriction sites were removed.


References