RNA
Part:BBa_K1941003
Designed by: Dimitri Coukos Group: iGEM16_EPFL (2016-10-13)
scCyc1_PP7
Scaffold RNA that targets the region c6 of CYC1 promoter and recruits the protein repressor module PCP-Mxi via the effector protein recruitments site PP7. PP7 is a well-characterized viral RNA sequence which is recognized by PCP protein. This RNA is composed of the dCas9-binding sgRNA that interacts with CYC1, and the PCP-binding RNA hairpin, PP7.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 180
Illegal NgoMIV site found at 209 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |