Coding

Part:BBa_K1898300

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 17:49, 18 October 2016 by Fionat17112752 (Talk | contribs) (→‎Gel Pictures)


CRYAA, crystallin alpha A

This gene codes for CRYAA, or crystallin alpha A, a crystallin protein that is more restricted to the lens.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]



Gel Pictures

We digested CRYAA with Xbal and Pst1 enzymes. The expected band size is ~700 bp. In the gel picture shown below, lane 1 is 1kb ladder and bands with the expected size are boxed in red:

Cryaa_pcr_gel_check.png

Sequencing

CRYAA DNA was sent to sequencing after it's moved into an iGEM backbone. The sequencing result confirms that CRYAA is right.

  • The four enzyme cutting sites are highlighted in red
  • CRYAA is highlighted in light blue

Cryaa.png

[edit]
Categories
//awards/part_collection/2016
Parameters
None