Coding

Part:BBa_K1898300:Design

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 18:04, 18 October 2016 by Fionat17112752 (Talk | contribs)


CRYAA, crystallin alpha A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the CRYAA DNA. Primers were designed to remove the stop codon and to move CRYAA into iGEM BioBrick. The primers are:

foward:


Source

The cDNA of CRYAA is ordered from OriGene

References