Generator

Part:BBa_K1890031

Designed by: Lycka Kamoen, Maria Vazquez   Group: iGEM16_TU_Delft   (2016-10-13)
Revision as of 09:34, 18 October 2016 by Lycka (Talk | contribs)


BolA gene with strong constitutive promoter, RBS and terminator

Introduction

BolA is a gene that controls the morphology of E. coli in the stress response (Santos, Freire, Vicente, & Arraiano, 1999). By overexpressing this gene at 37°C, naturally rod-shaped E. coli cells will become spherical (Aldea, Hernandez-Chico, De La Campa, Kushner, & Vicente, 1988). In this part, the gene is under control of a constitutive promoter, RBS and terminators.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 158
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None