Composite

Part:BBa_K1898150

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 09:45, 18 October 2016 by Fionat17112752 (Talk | contribs)


Strong promoter + Strong RBS + CH25H + 10x His tag + Double terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Gel Pictures

PCR was set up after moving the synthesized DNA from IDT to iGEM BIobrick. The expected PCR band sizes are ~1.3 kb.

Sequencing

The following sequence was sent to IDT for DNA syntehsis:


This quality report came back from IDT:

[edit]
Categories
//awards/part_collection/2016
Parameters
None