Part:BBa_K2016000

Strong constitutive E. coli promoter with included RBS - ready for cloning

BBa_K2016000 is a derivative of Bba_J23100, a promoter from a family of constitutive E. coli promoters isolated from a small combinatorial library. This family was submitted to the registry by Berkeley 2006. Sheffield 2016 has improved Bba_J23100 by adding an RBS downstream of the promoter to make it suitable for direct cloning.

Usage and Biology

Sheffield 2016 has used this promoter in order to design a biological device that responds to intracellular iron levels in bacteria. Bba_J23106 was also used in order to observe a difference between having a system under the control of a medium and a strong promoter.

Sequence and Features BBa_K2016000 is 91bp long, first 35 nucleotides make up a functional strong constitutive promoter for expression in E. coli, followed by a 50 nucleotide spacer (5' UTR) and ending with a 6 nucleotide ribosomal binding site (AGGAGG). Not understood