Coding

Part:BBa_K2074031

Designed by: Ruicheng Dai   Group: iGEM16_FAFU-CHINA   (2016-10-09)
Revision as of 09:40, 9 October 2016 by Registry (Talk | contribs)

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Cry11Aa(Codon optimization)+Extended FMDV 2A+Cyt1(Codon optimization)

cry11Aa and cyt1 are protein from Bacillus thuringiensis (or Bt),which have toxicity to the larve of mosquito.we use 2A system to express two protein at the same time in Chlamydomonas reinhardti.These two protein express at the same time can enhance the toxicity to the larve of mosquito.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 1681
    Illegal NgoMIV site found at 2029
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49
    Illegal BsaI.rc site found at 244
    Illegal BsaI.rc site found at 1459
    Illegal BsaI.rc site found at 2548
    Illegal SapI.rc site found at 1440


[edit]
Categories
Parameters
None