Coding
Part:BBa_K2074030
Designed by: Ruicheng Dai Group: iGEM16_FAFU-CHINA (2016-10-09)
Cry10Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)
cry10Aa and cyt2 are protein from Bacillus thuringiensis (or Bt),which have toxicity to the larve of mosquito.we use 2A system to express two protein at the same time in Chlamydomonas reinhardti.These two protein express at the same time can enhance the toxicity to the larve of mosquito.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 368
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 32
Illegal BglII site found at 2282 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 181
Illegal NgoMIV site found at 223
Illegal NgoMIV site found at 280
Illegal NgoMIV site found at 784
Illegal NgoMIV site found at 1460
Illegal NgoMIV site found at 2116
Illegal NgoMIV site found at 2416 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 202
Illegal SapI.rc site found at 924
[edit]
Categories
Parameters
None |