Coding

Part:BBa_K2074029

Designed by: Ruicheng Dai   Group: iGEM16_FAFU-CHINA   (2016-10-09)
Revision as of 09:28, 9 October 2016 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Cry10Aa(Codon optimization)+Extended FMDV 2A+Cyt1(Codon optimization)

cry10Aa and cyt1 are protein from Bacillus thuringiensis (or Bt),which have toxicity to the larve of mosquito.we use 2A system to express two protein at the same time in Chlamydomonas reinhardti.These two protein express at the same time can enhance the toxicity to the larve of mosquito.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
    Illegal NgoMIV site found at 223
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 784
    Illegal NgoMIV site found at 1460
    Illegal NgoMIV site found at 2116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 202
    Illegal BsaI.rc site found at 2635
    Illegal SapI.rc site found at 924


[edit]
Categories
Parameters
None