Coding

Part:BBa_K2074028

Designed by: Ruicheng Dai   Group: iGEM16_FAFU-CHINA   (2016-10-09)
Revision as of 09:24, 9 October 2016 by Registry (Talk | contribs)

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Cry4Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)

cry4Aa and cyt2 are protein from Bacillus thuringiensis (or Bt),which have toxicity to the larve of mosquito.we use 2A system to express two protein at the same time in Chlamydomonas reinhardti.These two protein express at the same time can enhance the toxicity to the larve of mosquito.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 986
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1378
    Illegal NgoMIV site found at 3631
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31
    Illegal BsaI.rc site found at 184
    Illegal BsaI.rc site found at 2071
    Illegal BsaI.rc site found at 2611
    Illegal BsaI.rc site found at 2920
    Illegal BsaI.rc site found at 3487
    Illegal BsaI.rc site found at 4150
    Illegal SapI.rc site found at 1188
    Illegal SapI.rc site found at 1230


[edit]
Categories
Parameters
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