Part:BBa_K1919303
livH
LivH is an integral membrane component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. LivH encodes a very hydrophobic protein with 7 or 9 predicted transmembrane regions. This device constructed in our team contains a promoter J23100, a RBS B0030 , livH coding region and a terminator B0010. The device can be transferred into E.coli and overexpresses livH directly. The expression of the livH RNA in the cell has been studied via qPCR. The quantified result suggests that compared with negative control E.coli (not cantain any plasmid), livH has been highly expressed in E.coli BL21 and E.coli DH5a.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 631
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 953
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 910
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