Coding

Part:BBa_K1965007

Designed by: Nik Franko   Group: iGEM16_Slovenia   (2016-10-17)
Revision as of 22:11, 17 October 2016 by Registry (Talk | contribs)

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His:CRY:Myc:nLuc

This part is one of the two components of the dimeryzing system which is induced by blue light. We adapted this system for the reconstitution of split luciferase to create a blue-light sensor. To the C-terminus of CRY, N-terminus of split firefly luciferase was added. Upon induction with light and concequent dimerisation, the split luciferase is reconstituted and regains activity, which results in biolumiescence if luciferin is added.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 411
    Illegal BglII site found at 870
    Illegal BamHI site found at 1349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1642
    Illegal NgoMIV site found at 2986
    Illegal NgoMIV site found at 3007
    Illegal AgeI site found at 295
    Illegal AgeI site found at 1024
    Illegal AgeI site found at 2710
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 647
    Illegal BsaI.rc site found at 56
    Illegal SapI.rc site found at 164
    Illegal SapI.rc site found at 2892


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Categories
Parameters
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