Composite
CiproR

Part:BBa_K1930004:Design

Designed by: Mareike Schmidt   Group: iGEM16_Groningen   (2016-10-16)
Revision as of 23:40, 16 October 2016 by Barawaclawik (Talk | contribs) (→‎References)


Ciprofloxacin resistance cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 536
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To design a ciprofloxacin resistance cassette we designed a gBlock that contains the Bacillus subtilis promoter PAtpI, which is active from a very early stage of germination and includes a ribosome binding site. The gBlock also contains the original qnrS1 gene sequence from E. coli, the double terminator BBa_B0015 from iGEM as well as the prefix and suffix for BioBricks. In summary the ciprofloxacin cassette consists of the following parts PAtpI+RBS+qnrS1+2TER.


Source

Ordered as gBlock. IDT (Integrated DNA technologies).

References

Gene ID

Quinolone resistance