Coding
scFv

Part:BBa_K1933002

Designed by: Tomoki Uchino   Group: iGEM16_Kyoto   (2016-10-10)
Revision as of 15:17, 17 October 2016 by Sora (Talk | contribs) (→‎Usage and Biology)


anti-Norovirus GII.4 scFv

This part codes for anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1].
This coding sequence was fused to INPNC-His (BBa_K1933001) or BclA-His (BBa_K1933000) in our project.
For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology


We used this part for construction of BBa_K1933200 and BBa_K1933201.

Characterization

Please see BBa_K1933200 and BBa_K1933201 for full characterization.

Reference

[1]Higo‐Moriguchi, Kyoko, et al. "Isolation of cross‐reactive human monoclonal antibodies that prevent binding of human noroviruses to histo‐blood group antigens." Journal of medical virology, 86.4, (2014): 558-567.

[edit]
Categories
//collections/immune_regulation/antibodies
//function/immunology
Parameters
biologyHomo sapiens
chassisE. coli