Coding
TetX

Part:BBa_K2150101:Design

Designed by: Zou Zhuoning   Group: iGEM16_UCAS   (2016-10-12)
Revision as of 17:43, 16 October 2016 by Zepeng Mu (Talk | contribs) (→‎Design Notes)


Tetracycline resistance protein from Bacteroides fragilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 562
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

protein sequence--gene sequence(codon preferance) 3 synonynous mutaions

Source

Tetracycline resistance protein from transposon Tn4351/Tn4400 found in Bacteroides fragilis

References

[1] Salis, H. M., Mirsky, E. A. & Voigt, C. A. Automated design of synthetic ribosome binding sites to control protein expression. Nat. Biotechnol. 27, 946–50 (2009).

[2] Espah Borujeni, A., Channarasappa, A. S. & Salis, H. M. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res. 42, 2646–2659 (2014).

[3] Engler, C., Kandzia, R. & Marillonnet, S. A one pot, one step, precision cloning method with high throughput capability. PLoS One 3, (2008).

[4] Gibson, D. G. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat. Methods 6, 343–5 (2009).