DNA

Part:BBa_K1951006:Design

Designed by: claire raynaud   Group: iGEM16_Aix-Marseille   (2016-10-11)
Revision as of 09:16, 11 October 2016 by Registry (Talk | contribs)

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FliC Desulfovibrio coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 400


Design Notes

We modified of the site Pst1 CTGCAG into CTGCGG. We added prefix and suffix subsequences containing EcoRI, XbaI and SpeI, PstI respectively.



Source

DNA sequence has been taken from the following website : http://www.ncbi.nlm.nih.gov/nucleotide/46451220?report=genbank&log$=nucltop&blast_rank=3&RID=WDB55ADB014

References