Composite

Part:BBa_K1997011:Design

Designed by: Xinyuan Qiu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 08:42, 13 October 2016 by Registry (Talk | contribs)

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P+R->sHRP-N->FRB->RBS->FKBP->sHRP-C->Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 383
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 625
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1553
    Illegal BsaI.rc site found at 908
    Illegal SapI.rc site found at 1667


Design Notes

dCas9 was codon optimized for expression in E.coli


Source

This part is assembled from K1789012, K1997000 and a dCas9 fragment


References