Coding
Part:BBa_K1983006:Design
Designed by: Vykintas Jauniškis Group: iGEM16_Vilnius-Lithuania (2016-09-15)
gp45 phenylalanine mutant M37 with C-terminal 6XHis-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 688
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed including NdeI restriction site for efficient cloning into expression vectors, XhoI site for cutting between the protein and 6XHis-Tag.
Source
T4 phage