Coding

Part:BBa_K1983004:Design

Designed by: Vykintas Jauniškis   Group: iGEM16_Vilnius-Lithuania   (2016-09-15)
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gp45 phenylalanine mutant M15 with C-terminal 6XHis-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed including NdeI restriction site for efficient cloning into expression vectors, XhoI site for cutting between the protein and 6XHis-Tag.


Source

T4 phage

References