Regulatory

Part:BBa_K2116011

Designed by: Asli Azizoglu   Group: iGEM16_ETH_Zurich   (2016-10-14)
Revision as of 15:20, 16 October 2016 by Aasli (Talk | contribs)


AND gate regulated by Nitric Oxide and 3OC6HSL

Promoter norV is the native promoter controlling the nitric oxide reduction operon (norRVW) in E. Coli. [1]

We created an AND gate by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox was added to create competitive binding between norR and esaR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2016
Parameters
None