Coding

Part:BBa_K1985006

Designed by: Jasmine Cornish   Group: iGEM16_Kent   (2016-10-14)
Revision as of 14:07, 16 October 2016 by JJECornish (Talk | contribs)

NOTOC__ mamO

MamO is involved in crystal nucleation.

The mamO construct in pSB1C3


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 493
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Usage: The mamO gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to start the process that should form magnetite (Fe(II)Fe(III)2O4) crystals when the cell is exposed to iron in solution. It was used to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.

Biology: MamO has been described as a crystal nucleation protein. It is part of the magnetosome of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.

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